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acclaim pepmap 100 c18 trap column  (Thermo Fisher)


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    Structured Review

    Thermo Fisher acclaim pepmap 100 c18 trap column
    Acclaim Pepmap 100 C18 Trap Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trapping+column+acclaim+pepmap+100/hypersil+gold+column/us12364411-499-10-19
    Average 90 stars, based on 1 article reviews
    acclaim pepmap 100 c18 trap column - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Injection:

    Article Title: Analysis of the N-glycosylation profiles of the spike proteins from the Alpha, Beta, Gamma, and Delta variants of SARS-CoV-2
    Article Snippet: Nanoflow liquid chromatography coupled to electrospray ionization tandem-mass spectrometry (LC–MS/MS) analysis was performed on an Orbitrap Eclipse Tribrid mass spectrometer and an UltiMateTM 3000 RSLCnano chromatography system (Thermo Scientific) using a signature ion triggered EThcD method as described previously [ , ]. .. Briefly, 8 μL of each sample (~ 0.5 mg protein) was injected on an integrated trapping column Acclaim PepMap 100, 75 μm × 2 cm length with 100% mobile phase A (0.1% formic acid in water) for trapping and separation was on an EASY-Spray C18 analytical column (Thermo Scientific, Cat#ES904, 75 μm i.d. ..

    Article Title: Mitochondria inside acute myeloid leukemia cells hydrolyze ATP to resist chemotherapy
    Article Snippet: Samples were subjected to nanoLC-MS/MS analysis using an UltiMate 3000 RSLCnano system (Thermo Fisher Scientific) coupled to a Q Exactive Plus Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) via a nanoelectrospray ionization source. .. For each injection, 4μL (1μg) of sample was first trapped on an Acclaim PepMap 100 20mm × 0.075mm trapping column (Thermo Fisher Scientific; 164535; 5μL/min at 98/2 v/v water/acetonitrile with 0.1% formic acid). .. Analytical separation was performed over a 95min gradient (flow rate of 250nL/min) of 4-25% acetonitrile using a 2μm EASY-Spray PepMap RSLC C18 75μm × 250mm column (Thermo Fisher Scientific; ES802A) with a column temperature of 45°C.

    Purification:

    Article Title: Protocol for differential multi-omic analyses of distinct cell types in the mouse cerebral cortex.
    Article Snippet: .. STAR Protocols 5, 102793, March 15, 2024 25 ll OPEN ACCESS Protocol 26 UPLC system (Dionex Ultimate 3000 Thermo Fisher Scientific, Bremen, Germany); Trapping column (for desalting and purification): Acclaim PepMap 100, 100 mm3 2 cm, 100 Å pore size, 5 mm particle size (Thermo Fisher Scientific, Bremen, Germany); Analytical column: Acclaim PepMap 100, 75 mm 3 50 cm, 100 Å pore size, 2 mm particle size (Thermo Fisher Scientific, Bremen, Germany)) coupled to a Quadrupole-Orbitrap-Iontrap Tribrid Mass Spectrometer (Orbitrap Fusion, Thermo Fisher Scientific, Bremen, Germany). a. ..

    Article Title: Protocol for differential multi-omic analyses of distinct cell types in the mouse cerebral cortex
    Article Snippet: .. This step measures TMT-labeled peptide fractions using a low pH RP-LC-MS method at a nano-UPLC system (Dionex Ultimate 3000 Thermo Fisher Scientific, Bremen, Germany); Trapping column (for desalting and purification): Acclaim PepMap 100, 100 μm × 2 cm, 100 Å pore size, 5 μm particle size (Thermo Fisher Scientific, Bremen, Germany); Analytical column: Acclaim PepMap 100, 75 μm × 50 cm, 100 Å pore size, 2 μm particle size (Thermo Fisher Scientific, Bremen, Germany)) coupled to a Quadrupole-Orbitrap-Iontrap Tribrid Mass Spectrometer (Orbitrap Fusion, Thermo Fisher Scientific, Bremen, Germany). a. ..

    Pore Size:

    Article Title: Protocol for differential multi-omic analyses of distinct cell types in the mouse cerebral cortex.
    Article Snippet: .. STAR Protocols 5, 102793, March 15, 2024 25 ll OPEN ACCESS Protocol 26 UPLC system (Dionex Ultimate 3000 Thermo Fisher Scientific, Bremen, Germany); Trapping column (for desalting and purification): Acclaim PepMap 100, 100 mm3 2 cm, 100 Å pore size, 5 mm particle size (Thermo Fisher Scientific, Bremen, Germany); Analytical column: Acclaim PepMap 100, 75 mm 3 50 cm, 100 Å pore size, 2 mm particle size (Thermo Fisher Scientific, Bremen, Germany)) coupled to a Quadrupole-Orbitrap-Iontrap Tribrid Mass Spectrometer (Orbitrap Fusion, Thermo Fisher Scientific, Bremen, Germany). a. ..

    Article Title: Protocol for differential multi-omic analyses of distinct cell types in the mouse cerebral cortex
    Article Snippet: .. This step measures TMT-labeled peptide fractions using a low pH RP-LC-MS method at a nano-UPLC system (Dionex Ultimate 3000 Thermo Fisher Scientific, Bremen, Germany); Trapping column (for desalting and purification): Acclaim PepMap 100, 100 μm × 2 cm, 100 Å pore size, 5 μm particle size (Thermo Fisher Scientific, Bremen, Germany); Analytical column: Acclaim PepMap 100, 75 μm × 50 cm, 100 Å pore size, 2 μm particle size (Thermo Fisher Scientific, Bremen, Germany)) coupled to a Quadrupole-Orbitrap-Iontrap Tribrid Mass Spectrometer (Orbitrap Fusion, Thermo Fisher Scientific, Bremen, Germany). a. ..

    Mass Spectrometry:

    Article Title: Protocol for differential multi-omic analyses of distinct cell types in the mouse cerebral cortex.
    Article Snippet: .. STAR Protocols 5, 102793, March 15, 2024 25 ll OPEN ACCESS Protocol 26 UPLC system (Dionex Ultimate 3000 Thermo Fisher Scientific, Bremen, Germany); Trapping column (for desalting and purification): Acclaim PepMap 100, 100 mm3 2 cm, 100 Å pore size, 5 mm particle size (Thermo Fisher Scientific, Bremen, Germany); Analytical column: Acclaim PepMap 100, 75 mm 3 50 cm, 100 Å pore size, 2 mm particle size (Thermo Fisher Scientific, Bremen, Germany)) coupled to a Quadrupole-Orbitrap-Iontrap Tribrid Mass Spectrometer (Orbitrap Fusion, Thermo Fisher Scientific, Bremen, Germany). a. ..

    Article Title: Protocol for differential multi-omic analyses of distinct cell types in the mouse cerebral cortex
    Article Snippet: .. This step measures TMT-labeled peptide fractions using a low pH RP-LC-MS method at a nano-UPLC system (Dionex Ultimate 3000 Thermo Fisher Scientific, Bremen, Germany); Trapping column (for desalting and purification): Acclaim PepMap 100, 100 μm × 2 cm, 100 Å pore size, 5 μm particle size (Thermo Fisher Scientific, Bremen, Germany); Analytical column: Acclaim PepMap 100, 75 μm × 50 cm, 100 Å pore size, 2 μm particle size (Thermo Fisher Scientific, Bremen, Germany)) coupled to a Quadrupole-Orbitrap-Iontrap Tribrid Mass Spectrometer (Orbitrap Fusion, Thermo Fisher Scientific, Bremen, Germany). a. ..

    other:

    Article Title: STING induces HOIP-mediated synthesis of M1 ubiquitin chains to stimulate NF-κB signaling.
    Article Snippet: STING activation by cyclic dinucleotides induces IRF3and NF-κBmediated gene expression in mammals, as well as lipidation of LC3B at Golgi-related membranes.. While mechanisms of the IRF3 response are well understood, the mechanisms of NF-κB activation via STING remain unclear.. We report here that STING activation induces linear/M1-linked ubiquitin chain (M1-Ub) formation and recruitment of the LUBAC E3 ligase, HOIP, to LC3B-associated Golgi membranes where ubiquitin is also localized.

    Article Title: STING induces HOIP-mediated synthesis of M1 ubiquitin chains to stimulate NF-κB signaling
    Article Snippet: For each analysis, ~1 μg of the tryptic digest was loaded and desalted in an Acclaim PepMap 100 trapping column (75 μm, 2 cm) at 4 μL/min for 5 min. Peptides were then eluted into a Thermo Scientific Acclaim PepMapTM 100 column, (3 μm, 100 Å, 75 μm × 250 mm) and chromatographically separated using a binary solvent system consisting of A: 0.1% formic acid and B: 0.1% formic acid and 80% acetonitrile at a flow rate of 300 nL/min.

    Multiplex sample analysis:

    Article Title: Protocol for differential multi-omic analyses of distinct cell types in the mouse cerebral cortex
    Article Snippet: .. This step measures TMT-labeled peptide fractions using a low pH RP-LC-MS method at a nano-UPLC system (Dionex Ultimate 3000 Thermo Fisher Scientific, Bremen, Germany); Trapping column (for desalting and purification): Acclaim PepMap 100, 100 μm × 2 cm, 100 Å pore size, 5 μm particle size (Thermo Fisher Scientific, Bremen, Germany); Analytical column: Acclaim PepMap 100, 75 μm × 50 cm, 100 Å pore size, 2 μm particle size (Thermo Fisher Scientific, Bremen, Germany)) coupled to a Quadrupole-Orbitrap-Iontrap Tribrid Mass Spectrometer (Orbitrap Fusion, Thermo Fisher Scientific, Bremen, Germany). a. ..



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